The installer places shortcuts to bd facsdiva software, bd facsdiva data manager, the bd facsdiva software. Regarding this runtime module, you are prohibited from. Bd facsdiva v6 software is a collection of rich tools for flow cytometer and application setup. See dragging gates into the population hierarchy on page 232 in the bd facsdiva software reference manual.
After a successful login, the bd facsdiva workspace appears showing the main application windows which include browser, cytometer, inspector, acquistion dashboard and global worksheet. If you are not familiar with the windows operating system, see the documentation provided with your computer. Bd biosciences has developed a protocol for enabling application settings on machines running the facsdiva v6 software, to create settings that allow for standardized consistent fluorescence intensity target values to be obtained across experiments run on different days or instruments. This automated package will help scientists attain the best performance and most consistent data from bd flow cytometry systems. The letter also stated that bd biosciences rep will contact the users when the new software is available. Bd intro to flow training about 1 hour bd facsdiva v6 software overview course 6. Unc flow cytometry core facility lsr ii operation 2018 7 data displayed in a normal worksheet is not dependent on where the current tube pointer is set. All baselines, performance checks, and application settings will have to be recreated, as needed. Facscalibur self user training research ut health san antonio. Introduction to flow cytometry introduction to bd facsdiva. Bd accuri c6 plus installation and operator training. The bd facsdiva software reference manual contains reference information on all software components. The university of texas health science center flow cytometry. Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2.
Bd facsdiva v6 software is a collection of rich tools for flow cytometer and application setup, data acquisition, and analysis. Bd facscanto ii flow cytometer and bd facscanto clinical software faqs. Compensation go to experiment menucompensation setupcreate compensation controls. Interference there are no known incompatibilities with bd facscanto clinical. Bd biosceinces instrument and application trainings. The cytometer setup and tracking features in bd facsdiva v6. Bd facscanto 10color clinical flow operator with tube loader 4 days. Learn to use bd facsdiva v6 software with our free 6. At the facility, the investigators can use the following programs for data analysis. Since data exported from the bd facsdivat software except fcs 2 files is saved in linear format, the user must tell flowjo in which format to display the data log or linear. This allows users to select the correct configuration for their work. Bd biosciences will release a version of bd facsdiva software to correct this problem. For the demonstration, we will assume this is a 4color flow cytometric analysis. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5.
Completion of a bd operator course for a digital cytometer using bd facsdiva software v6 or equivalent experience on a bd digital cytometer. Using bd facs diva and accuri importers in fcs express v6. Bd facsdiva v6 software overview course for bd digital instrumentation flow cytometry courses verity software house fcs express software, and support for more information about fcs express availability at iu bloomington, visit the data analysis page. Bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data. Bd facslink lis interface solution with bd facscanto clinical software bd biosciences. For example, the plots in the graphics below display data from tubes 001 and 002. Software packages available flow cytometry core facility. To access the pdf, choose start programs bd facsdiva software. The following sections contain an overview of these controls. With 4 lasers, 14 color detectors, and ability to detect particles as small as 200 nm using the forward scatter detector. In addition to the standard configuration shown above, lsrfortessa can be easily reconfigured to detect yfp and gfp in the same sample. Bd facsdiva software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data.
If your last name is shorter than 3 letters, your login will be your full first. Modfit lt analysis software verity software house is available upon request. The instruments use fluoresence activated cell sorting facs technology to count and examine cells and dna and provide simultaneous analysis of. Note that because of a change in the beads for bd facsdiva software v7. Bd facslink lis interface solution with bd facsdiva software. In addition, compensation adjustment for each channel was performed using single stained compensation beads bd biosciences.
If you are an experienced user, try the updated tutorials to become familiar with new software features. Getting started guide and reference manual pdf files, and the readme file on the desktop. The online help system contains the bd facsdiva software reference. Bd facsdiva software quick reference guide for bd facscanto systems with loader option work. Upcoming click on the the blue link to register for the event past click on the blue link to view the presentation. Student needs to understand some flow cytometry theory before the first session. New feature to apply gate coordinates such as shape and size between worksheets to other tubes in an open experiment. Invitrogen introduction to flow cytometry 15 minutes bd bioscience training courses. Jan 26, 2017 using bd facs diva and accuri importers in fcs express v6. Bd cytometer setup and tracking beads are for research use only with bd facsdiva software v6. Evaluating fluorescence sensitivity on flow cytometers. Upload certificates of completion to ppms with your training request. Bd facsdiva software v6 with spa ii and lwa sample preppers bd facsdiva software v6 with spa iii and lwa sample preppers bd facsdiva software v6.
Flow cytometry analysis software research and innovation. It is equipped with 5 lasers and 18 color detectors. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. Bd biosciences, a segment of bd becton, dickinson and company, today announced the introduction of bd facsdiva 6. Bd facscanto ii system overview bd facsdiva software v6 bd facsdiva software v6 overview. Facsdiva v6 software overview course bd biosciences accuri c6 software user guide. Learn about the software application notes and technical bulletins. Feb 12, 2015 importers allow you to use your plots and gates from acquisition as a starting point for your analysis. See headers and footers on page 100 in the bd facsdiva software reference manual. Unc flow cytometry core facility lsrfortessa operation 2017 3. May 17, 20 bd biosciences has developed a protocol for enabling application settings on machines running the facsdiva v6 software, to create settings that allow for standardized consistent fluorescence intensity target values to be obtained across experiments run on different days or instruments. Unc flow cytometry core facility lsr ii operation 2018 4. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers. Student must agree to schedule these 3 sessions within a twoweek period.
The flow cytometry core provides the ucla research community with flow cytometry instrumentation and expertise in cell analysis and cell sorting services. Along with builtin database and database utilities, bd facsdiva software provides automated data export in a variety of formats including xml, fcs, and even zip, for multiple files concurrently. Development and validation of flow cytometry methods for. Course prerequisites basic computer skills are required for the course duration. Each vial of beads contains equal concentrations of beads of. Moflo astrios eq moflo astrios is a high speed cell sorter that can be outfitted with different nozzle sizes 70 and 100. The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation.
Displaying facsdiva data and using the facsdiva importer. It is available as a pdf that can be opened, searched, and printed using adobe acrobat reader. User are asked to contact bd customer support center at 18772328995 if they have any additional questions. Application notes and technical bulletins learn more about reagent selection and instrument setup with our application notes and technical bulletins. Sophie novault bd facs diva software research institut. The beads allow the software to automatically characterize, track, and report measurements of supported bd digital flow cytometers. Ddx4positive and negative cells, for both human ovarian cells and transfected hek 293 t cells, were sorted using an aria iiu cytometer equipped with a 488 nm laser, running bd facsdiva v6 software bd biosciences, oxford, uk.
Preparing samples 1 perform daily inspection and startup for the spa ii and startup for the lwa. Bd facscanto ii resources cell analyzer research instrument. Sorting and analysis scheduling is done through ppms. Application setup across multiple flow cytometers using bd facsdiva v6 march 2012. Appendix a technical overview this appendix provides more information about. At least, 250,000 events were acquired per sample using bd facsdiva v6.
Bd facsdiva software overview selfpaced data display in bd facsdiva software selfpaced using the worksheet window in bd facsdiva software selfpaced using templates in bd facsdiva software selfpaced bd facsvia online training program. See applying gate coordinates on page 240 in the bd facsdiva software reference manual. Activation and polarization of circulating monocytes in. Why waste effort recreating your work, when you can g. Importing bd facsdiva and accuri experiments youtube. Bd facsdiva v6 software overview course for bd digital instrumentation flow cytometry courses verity software house flowjo software, and tutorials and manuals for more information about flowjo availability at iu bloomington, visit the data analysis page. That means that even though two plots may look similar, one may have to closely inspect axes to see if the axes vary widely from plot to plot. The factory default flowjo preference is to show all fluorescence parameters in log and both side scatter and forward scatter in linear format. Bd facscanto ii clinical flow operator with tube loader and sample preparation instruments 4. Bd facsdiva software contains vxworks as embedded software runtime module. Most software functions are controlled using the tool bar at the top of the workspace and toolbars within the browser and worksheet windows. Introduction to flow cytometry introduction to bd facsdiva software v6 overview refreshertraining.
Bd biosciences instrument and application trainings. The runtime module was developed by a third party and we are obligated to notify our customers about the limitations for use. See using the worksheet grid tab on page 186 in the bd facsdiva software reference manual. Isolation, cultivation and characterization of adult. The bd cytometer setup and tracking application guide is available as a pdf that can be opened, searched, and printed using adobe acrobat reader. To visualize the colonies for overview images, aspirate pregm and. All other cytometer and fluidics cart functions are controlled by bd facscanto clinical software and bd facsdiva software provided with the instrument. The bd facsdiva software quick start guide contains tutorials designed to familiarize you with bd facsdiva software. Bd facsdiva software standardizing application settings across multiple cytometers.
These shortcuts are also added to the start menu start programs bd facsdiva software. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and instruments. Using application settings to standardize flow cytometry. Firsttime users of bd facsdiva software should read. This automated package will help scientists attain the best performance and most consistent data from bd flow cytometry systems used for important basic and clinical research. Cells were sorted on high purity setting at no more than 5000 eventss using a 70 m nozzle at 55 psi with a 90 hz. Cytometers with hts option bd facsdiva software v6. Unc flow cytometry core facility lsrfortessa operation 2017 7 data displayed in a normal worksheet is not dependent on where the current tube pointer is set. Bd cytometer setup and tracking application guide trigenotoul. For assisted analysis, contact a flow cytometry operator directly to schedule an appointment. These new beads also add additional support to over 100 different optical configurations for the bd facscanto, bd facsaria, and bd. Table 22 provides a brief overview of window components. Development and validation of flow cytometry methods for pharmacodynamic clinical biomarkers.
Bd facsdiva software provides a graphic of the cytometers physical optical configuration, showing the layout of detectors, filters, and mirrors. For software troubleshooting, refer to the bd facscanto clinical software reference manual, the bd facsdiva software reference manual, or the bd facscanto instructions for use. These are oneonone instrument operation sessions with flow core staff. Bd facsdiva software resources software bd biosciences. Troubleshooting the tips in this section can help you troubleshoot issues that might arise when using this cytometer. Refer to the information supplied by the manufacturer for applicationspecific limitations. If you are a new user, use the tutorials to get started with the software. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. To access the bd facsdiva software reference manual, choose help software manual or doubleclick the shortcut icon on the desktop.
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